Mycotoxins constitute a major heath and economic concern. We installed HPLC/UV or HPLC/fluorescence methods for quantification of mycotoxins Aaflatoxins (AF B1 & M1), Zearalenone (ZEA), and Deoxynivalenol (DON)). The method was used for screening dose/response trapping capacity of adsobents (Bentonite, activated charcoal and a biochar obtained from pine). Caco 2 cells ware used for evaluating toxicity and effects of the three adsorbents, at wide mass ratios, on AF M1 intestinal absorption. Bentonine and the biochar sample exhibited saturation curves with all the mycotoxins over mass ratios from 10 to 3000. The effects on AF M1 absorption by Caco-2 cells behaved accordingly, but bentonite was found to be toxic to Caco-2 cells. Activated charcoal exhibited a much higher capacity to trap mycotoxins (100% adsorption of the 4 mycotoxins at the lowest mass ratio here tested (10 :1, Adsorbent/M) ; and it is not toxic to Caco-2 cells at the tested doses.Biochars from biomass byproducts in general, and from food waste in particular, therfore seem to be valuable sustainable remediation materals against mycotoxins. The investigations will be continued following the same methodology, improved if needed, and using well characterized food waste biochars.

Nardjes Benbernou, El Hassan Ajandouz, Marc Maresca, Viviane Robert; Aix Marseille Univ, CNRS, Centrale Marseille, iSm2, 13013 Marseille, France